his抗体说明书

His标签抗体（小鼠单克隆抗体）产品信息
Product Name
His-Tag Mouse Monoclonal Antibody
Host Species
Mouse
Purification
>95% Protein A purified
Applications
WB / ELISA / IF
Specificity
His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal,or N-terminal 6His-tagged proteins.
Immunogen Type
Peptide-KLH
Ig class
Immunogen-specific mouse IgG1
Content
1.0 mg/ml with PBS, pH 7.4.
Storage
For up to 12 months at -80℃. After receivice, the antibody should be divided into the right amount .Avoid repeated freezing and thawing cycles.
稀释比
WB：1:2500~1:5000
ELISA: 1:2500~1:10000
IF :1:500
最佳浓度/稀释比应根据实验决定
示例
Western Blot analysis of HHHHHH tagged-fusion protein
Lane 1: C-His fusion protein
Lane 2: N-His fusion protein
Lane 3: Internal-His fusion protein
M: Molecular weight markers
Using His-Tag Mouse Monoclonal Antibody
(Dilute Proportion: 1:3000)

Western Blot analysis of HHHHHH tagged-fusion protein
Lane 1: C-His fusion protein
Lane 2: N-His fusion protein
Lane 3: Internal-His fusion protein
M: Molecular weight markers
Using His-Tag Mouse Monoclonal Antibody
(Dilute Proportion: 1:5000)

Western Blot analysis of HHHHHH tagged-fusion protein
Lane 1: C-His fusion protein
Lane 2: N-His fusion protein
Lane 3: Internal-His fusion protein
M: Molecular weight markers
Using His-Tag Mouse Monoclonal Antibody
(Dilute Proportion: 1:15000)